Journal: BMC Cardiovascular Disorders

Article Title: Circular RNA expression profile and its potential regulative role in human abdominal aortic aneurysm

doi: 10.1186/s12872-020-01374-8

Figure Lengend Snippet: circRNAs expression profiles detected by microarray in the AAA group and control group. a The box plot shows the nearly identical distributions of normalized intensity values from the aortic samples of the AAA and control group. b The scatter plot is built to assess the expression variation of circRNAs between the two groups. The X and Y axes indicate the normalized intensity values of each circRNAs from the AAA and control group. The dots above the upper green line and below the lower green line represent the dysregulated circRNAs with a fold change (FC) > 2.0 between the two groups. c The volcano plot presents differentially expressed circRNAs in AAA. The vertical lines correspond to 2-fold upregulation and downregulation, the horizontal line indicates P value of 0.05. The red dots represent the differentially expressed circRNAs (FC > 2.0 and P value < 0.05). d Hierarchical clustering analysis reveals a distinguishable expression profile of circRNAs between the AAA and control group. Each column indicates an aortic sample, each row represents a circRNA. The red and green color indicate high and low expression level, respectively. e Chromosomal distribution of the differentially expressed circRNAs between the two groups

Article Snippet: The fragmented labeled cRNAs were hybridized onto the circRNA expression microarray slide (Arraystar Human circRNA Array V2).

Techniques: Expressing, Microarray, Control

Journal: BMC Cardiovascular Disorders

Article Title: Circular RNA expression profile and its potential regulative role in human abdominal aortic aneurysm

doi: 10.1186/s12872-020-01374-8

Figure Lengend Snippet: Validation of six randomly selected dysregulated circRNAs by qRT-PCR. Each circRNA was evaluated at least three times and compared with the results of microarray. The Y axis indicates the fold change of AAA vs control of each circRNA

Article Snippet: The fragmented labeled cRNAs were hybridized onto the circRNA expression microarray slide (Arraystar Human circRNA Array V2).

Techniques: Biomarker Discovery, Quantitative RT-PCR, Microarray, Control

Journal: BMC Cardiovascular Disorders

Article Title: Circular RNA expression profile and its potential regulative role in human abdominal aortic aneurysm

doi: 10.1186/s12872-020-01374-8

Figure Lengend Snippet: The predicted circRNA/miRNA interaction networks for six randomly selected circRNAs. a , b The red nodes indicate upregulated circRNAs. c - f The blue nodes represent downregulated circRNAs. The green nodes are five complementary binding miRNAs of each circRNA

Article Snippet: The fragmented labeled cRNAs were hybridized onto the circRNA expression microarray slide (Arraystar Human circRNA Array V2).

Techniques: Binding Assay

Journal: Haematologica

Article Title: The process of somatic hypermutation increases polyreactivity for central nervous system antigens in primary central nervous system lymphoma

doi: 10.3324/haematol.2019.242701

Figure Lengend Snippet: Expression of SNRPC in primary lymphoma of the central nervous system (PCNSL). (A) Nearly all CD20+ (FITC) tumor cells of PCNSL show a nuclear expression of SNRPC (Cy3). (B) Intermingled with tumor cells of a PCNSL, single CD68+ (FITC) macrophages also express SNRPC (arrows, Cy3); additionally, there are also SNRPC-negative CD68+ macrophages (arrowheads). (C) Cortical NeuN+ (FITC) neurons express SNRPC (arrows, Cy3). (D) Strongly activated GFAP+ (FITC) reactive astrocytes express SNRPC (arrows, Cy3). (E) In the white matter, some Olig2+ (FITC) oligodendrocytes express SNRPC (arrows, Cy3). (F) CD34+ (FITC) endothelial cells of neocortical cerebral blood vessels do not express SNRPC (Cy3). (G) mRNA expression of SNRPC recognized on the ProtoArray in the normal central nervous system (CNS) and PCNSL. SNRPC is downregulated CNS and upregulated in PCNSL samples. The line represents the SNRPC tag on the U95Av2 microarray (Affymetrix) in individual samples of ten normal CNS tissues and 21 PCNSL published previously.15 SNRPC was significantly differentially expressed between CNS and PCNSL ( P <0.01, Student t -test). (A-F) Double immunofluorescence with rabbit anti-SNRPC (clone EPR16034, Abcam, Cy3) and mouse anti-CD20 (clone L26, DCS, FITC in A), mouse anti-CD68 (clone KP1, DCS, FITC in B), mouse anti-NeuN (clone A60, Millipore, FITC in C), mouse anti-GFAP (clone GA-5, Biogenex, FITC in D), mouse anti- Olig2 (clone Olig2/2400, Abcam, FITC in E), and mouse anti-CD34 (cloneQBend/10, Biogenex, FITC in F). Microphotographs were taken with an Axiophot (Zeiss, Jena, Germany) and Zen 2 software (Zeiss). Original magnification: x400 (objective: x40). Inserts: x750 (A-C), x1000 (D-F). Overlay of the microphotographs and adjustment for contrast and brightness were performed with Adobe Photoshop software version CC.

Article Snippet: Protein microarray slides (9374 human proteins, ProtoArray, Thermo Fisher Scientific) including internal controls were analyzed as described previously., ,

Techniques: Expressing, Microarray, Immunofluorescence, Software